Institute of Medical Physics, Biophysics, Informatics and Telemedicine

Sasinkova 2, III. floor, 813 72 Bratislava

 

Head of the Institute:    doc. RNDr. Martin K o p á n i, PhD. 

tel. +421 2 90119 530    

e-mail: martin.kopanifmed.uniba.sk , fyzika.prednostafmed.uniba.sk

 

Sekretary:  Vlasta Andrášová

tel. 02/90119 336, e-mail:  fyzika.sekretariatfmed.uniba.sk, vlasta.adrasovafmed.uniba.sk 

 

 

Information about Institute

      At the Department of physics, biophysics, informatics and telemedicine, we are interested in several research fields. One of the topics we focus on is diagnostics of obesity in adolescents using anthropometric measurements, and the importance of standard and nonstandard biochemical markers in patophysiology of cardiometabolic diseases. We are also involved in study of the impact of unhealthy diet on organism in animal models. Specifically, we pay attention to AGEs – advanced glycation end products. Our research includes study of the mechanisms of a treatment modality for oncologic diseases - photodynamic therapy (PDT) that combines effects of visible light and photosensitizing agent, localizing predominantly in tumor tissue, and identification of influencing factors.

 

Fluorescence images of a 3D collagen culture of an esophageal carcinoma cell line a) untreated, b) after aminolevulinic acid-based PDT. The cultures were incubated with fluorescent dyes enabling visualisation of intracellular organels (Acridin Orange - green fluorescence (nuclear structures, cytoplasm), CellMask Orange - orange fluorescence (cell membrane).
Fluorescence images of a 3D collagen culture of an esophageal carcinoma cell line a) untreated, b) after aminolevulinic acid-based PDT. The cultures were incubated with fluorescent dyes enabling visualisation of intracellular organels (Acridin Orange - green fluorescence (nuclear structures, cytoplasm), CellMask Orange - orange fluorescence (cell membrane).

 

     In addition, we seek to develop physiologically relevant in vitro tumor models, taking into account complexity and specificity of tumor tissues, in the form of 3D cell cultures. The main experimental methods applied in our research include optical microscopy and UV-VIS spectroscopy.

     We deal also with application of various physical methods in study of cells and tissues, mainly from the view of presence of metals (iron, copper) and others, for living body foreign substances, which accumulate in the body for certain reasons (metabolic diseases, environmental factors, ...). We study localisation, distribution, structure and magnetic properties of iron in some brain tissues -  substantia nigra and globus pallidus with histological and immunohistological methods. We used both light microscope (polarization, fluorescence) and electron microscope (scanning and transmission) microscopes with energy-dispersive microanalysis and electron diffraction. For investigation of magnetic properties we used Superconducting Quantum Interference Device (SQUID) magnetometry and Mössbauer spectroscopy.  

Human brain, globus pallidus. Blue dyed deposits of Fe(III) ions. Perls’ reaction, Left: x 20. Right: glioma x 40.
Human brain, globus pallidus. Blue dyed deposits of Fe(III) ions. Perls’ reaction, Left: x20. Right: glioma x40.

 

     Moreover, we deal with the realization of animal experiments for monitoring of cognitive functions, such as: Morris water maze, the open field test, the light/dark preference test, the elevated plus maze test, the forced swim test and the social interaction test. One investigation area of Institute of Medical Physics, Biophysics, Informatics and Telemedicine is also characterization and design of novel sensor and biomonitoring systems. We are part of multidisciplinary research in field of human physiology, psychology and sensor systems.

 

Staff

Mgr. Ján Pánik, PhD.

Comenius University in Bratislava, Faculty of Medicine, Institute of Medical Physics, Biophysics, Informatics and Telemedicine
vedecký (výskumný a vývojový) zamestnane
+421 2 9011 9859

Mgr. Tibor Teplický, PhD.

Comenius University in Bratislava, Faculty of Medicine, Institute of Medical Physics, Biophysics, Informatics and Telemedicine
vedecký (výskumný a vývojový) zamestnane
M 10, STÚ 3. poschodie
+421 2 9011 9928

doc. Ing. Erik Vavrinský, PhD.

Comenius University in Bratislava, Faculty of Medicine, Institute of Medical Physics, Biophysics, Informatics and Telemedicine
vedecký (výskumný a vývojový) zamestnane
+421 (2) 60 291 655

Marianna Kupcová

Comenius University in Bratislava, Faculty of Medicine, Institute of Medical Physics, Biophysics, Informatics and Telemedicine
laborant v biológii a príbuzných odboroc
+421 2 9011 9332

Andrea Račeková

Comenius University in Bratislava, Faculty of Medicine, Institute of Medical Physics, Biophysics, Informatics and Telemedicine
laborant v biológii a príbuzných odboroc

Magdaléna Vargová

Comenius University in Bratislava, Faculty of Medicine, Institute of Medical Physics, Biophysics, Informatics and Telemedicine
umývačka

Monika Kausitzová

Comenius University in Bratislava, Faculty of Medicine, Institute of Medical Physics, Biophysics, Informatics and Telemedicine
pomocník a upratovač v kancelárii
1 [2]